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Plant Cell Analysis with Microfluidics: High-Throughput Screening of Single Plant Cells

Summary

Learn how high-throughput microfluidic technology enables single-cell analysis and sorting of plant cells at scale, advancing plant biotechnology research. This study introduces a novel droplet-based platform that can encapsulate and analyze over 100,000 plant protoplasts per hour, quantifying gene expression responses and enabling the selection of transgenic cells based on fluorescence. This approach has vast potential for genetic research, allowing efficient screening of plant phenotypes and synthetic circuits with minimal plant material.

Research highlights

Study Title: Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi-step assurance of monoclonality.
Authors: Pybus, L. P., Kalsi, D., Matthews, J. T., Hawke, E., Barber, N., Richer, R., Young, A., & Saunders, F. L.
Published In: Biotechnol. J., 17, e2100357, 2022
DOI: 10.1002/biot.202100357

Key findings

High-throughput technology: Cyto-Mine® offers high-throughput capabilities that allow for the screening of a large number of single cells and finding the best ones for biomanufacturing.
Precise dispensing and imaging: The precise dispensing and integrated imaging functionalities provide unique opportunities for improved monoclonality assurance.
Reliable and reproducible workflows: The robust and efficient workflows ensure that users can consistently generate cell lines that meet regulatory requirements.

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