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Accelerating Monoclonal Cell Line Development with Picodroplet Technology

Summary

This publication highlights an innovative approach for creating biomanufacturing-ready cell lines using Cyto-Mine® technology. By coupling microfluidic picodroplet workflows with integrated plate imaging, researchers achieved over 99% assurance of monoclonality, improving efficiency, and meeting stringent regulatory standards. Cyto-Mine®’s ability to screen, sort, and dispense single cells with high precision ensures a reliable and reproducible cell line development process, ideal for biotherapeutic applications.

Research highlights

Study Title: Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi-step assurance of monoclonality.
Authors: Pybus, L. P., Kalsi, D., Matthews, J. T., Hawke, E., Barber, N., Richer, R., Young, A., & Saunders, F. L.
Published In: Biotechnol. J., 17, e2100357, 2022
DOI: 10.1002/biot.202100357

Key findings

High-throughput technology: Cyto-Mine® offers high-throughput capabilities that allow for the screening of a large number of single cells and finding the best ones for biomanufacturing.
Precise dispensing and imaging: The precise dispensing and integrated imaging functionalities provide unique opportunities for improved monoclonality assurance.
Reliable and reproducible workflows: The robust and efficient workflows ensure that users can consistently generate cell lines that meet regulatory requirements.

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